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Image Search Results
Journal: Cells
Article Title: Impact of Progerin Expression on Adipogenesis in Hutchinson—Gilford Progeria Skin-Derived Precursor Cells
doi: 10.3390/cells10071598
Figure Lengend Snippet: Isolation of SKPs from control and HGPS fibroblasts. ( a ) Panel showing the protocol for SKP isolation. Briefly, fibroblasts were pelleted and treated with HBSS buffer (pH 5.7) for 30 min at 37 °C. Cells were cultured in SKP media containing DMEM low glucose, EGF, FGF, and B27. The flasks were agitated daily, and the spheroids were harvested at day 4 for analysis. ( b ) SKP formation from both control (GMO1651c, GMO5565) and HGPS (HGADFN127, HGADFN003) fibroblasts with 5 and 30% senescence (SNS). ( c , d ) Quantification of the number and the diameter of the spheroids from control and HGPS fibroblast cultures with 5 and 30% SNS at day 4. Values are presented as mean ± SD ( n = 3), not significant (ns), * p < 0.05, ( c , d ) unpaired t -test. HBSS: Hank’s Balanced Salt Solution, DMEM: Dulbecco′s modified Eagle medium, EGF: epidermal growth factor, FGF: fibroblast growth factor, SKPs: skin-derived precursor cells, SNS: senescence.
Article Snippet: The human primary dermal fibroblast cell lines,
Techniques: Isolation, Control, Cell Culture, Modification, Derivative Assay